Room Temperature
The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.
Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
5.6 lbs
12.00 x 10.50 x 8.00
