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EZ-96 DNA Methylation-Gold Kit, Deep-Well, 2 x 96 Rxns

Price$ 526.77
  • SKU: ZD5008
  • Pack Size: Deep-Well
Quantity
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EZ-96 DNA Methylation-Gold Kit, Deep-Well, 2 x 96 Rxns

Room Temperature


The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to reduce incubation time and ensure conversion rates >99%. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

High-throughput (96-well) bisulfite conversion of DNA in less than 3 hours. A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate.
Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.

2.4 lbs

9.00 x 6.50 x 6.00



Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.  Recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion: >99%
Elution Volume: ≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well
Equipment: Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers
Input: 500 pg - 2 µg of DNA.
Processing Time: 3 hours
Recovery: >75%
Sample Source: Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

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