Water with Heat
Room Temperature
Simmons Citrate Agar is a selective and differential medium used to determine an organism’s ability to utilize citrate as the sole carbon source and ammonium ions as the sole nitrogen source. This medium was developed to differentiate among the Enterobacteriaceae.
The formulation contains sodium citrate as the only available carbon source, ammonium dihydrogen phosphate as the nitrogen source, and the pH indicator bromothymol blue. When an organism can metabolize citrate, it produces alkaline byproducts that increase the pH, changing the medium from green to blue. Growth with a color change is considered a positive result.
Simmons Citrate Agar is primarily employed to distinguish Gram-negative, oxidase-negative bacteria, particularly separating Enterobacter, Klebsiella, and Citrobacter species (citrate-positive) from Escherichia coli and other citrate-negative organisms. Its selectivity arises from the fact that only organisms capable of transporting and metabolizing citrate under these defined conditions will grow.
1.2 lbs
3.50 x 7.00 x 3.50
Research or further manufacturing use only, not for food or drug use.
